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On line tutorials
The following are tutorials I created to walk you through concepts and problems. I strongly recommend that you watch the first set of tutorials (gold background) as they are directly relevant to class materials. The second set (gray background are general interest tutorials of lesser importance to exam and quiz performance. The tutorials I created for the Lehninger text are also useful.
To access the tutorials I created for Lehninger Principles of Chemistry, 5th edition, click on this link. You will need to enter a username and password for access. They are case sensitive, enter them exactly like this (all lower case, no spaces)
username: ensigns
password: tadpoles
1. General strategy for working buffer problems 2. Buffer
problem example 1: You have a 0.1 M solution
of a weak acid and it’s conjugate base ([HA] + [A-] =
0.1 M) buffered at pH 7.4. The pKa for the weak acid is 6.8.
What are the concentrations of HA and A- in this solution? 3. Buffer problem example 2: You need to prepare 0.5 liters of a 0.2 M solution of MOPS (Morpholino-propanesulfonic Acid) buffer with a pH of 7.6 at 25 C°. The pKa for MOPS buffer is 7.20. What mass of MOPS free acid (the “HA” form of MOPS, Mr = 209.3) and what volume of 6M NaOH must be combined with water to make this solution properly? 4. Protein purification tutorial NEW: Meaning of enzyme activity, how to interpret and fill in a purification table, calculating enzyme subunit composition, etc. Broken into chpaters so you can skip to parts of interest. 5. Edman degradation/peptide sequencing tutorial: This tutorial should provide you the tools/knowledge you need to solve the quiz problems involving reagents in protein analysis and peptide sequencing. Now in flash format for superior viewing pleasure. 6. Kinemage overview tutorial: How to download the kinemage and prekin software. How to download and view kinemage files. 7. Kinetics tutorial 1: Steady state assumption, how enzyme assays are conducted 8. Kinetics tutorial 2: Derivation of the Michaelis-Menten Equation 9. Kinetics problem solving 1: Fall 2005 exam 1, problem 8 10. Kinetics problem solving 2: Fall 2003 exam 1, problem 6 11. Thermodynamics tutorial 1: Adding two reactions with known free energies to get free energy of reaction; converting standard free energy change to equilibrium constant; calculating free energy change under nonstandard conditions 12. Thermodynamics tutorial 2: The infamous quiz question on the ratio of creatine to phosphocreatine 13. Oxidation numbers tutorial: How to calculate oxidation numbers for bioorganic molecules- important in electron "bookkeeping" for oxidative metabolism |
Tutorials you may find interesting: 1. Downloading PDB files and converting to kinemages: How to download a PDB file from the Protein DataBank, and convert the PDB file into a kinemage using the free "prekin" software. 2. Using the KING plugin at the protein databank: This is a pretty cool tool for quickly viewing protein structures, right in your web browser, using the next generation kinemage plugin. 3. PyMol: a quick tutorial: How to download and use PyMol, the most powerful protein visualization software available. And it's FREE!! |
Tutorial/solutions for the 2006 final exam
Part 1: questions 1-25 (new material)
Part 2: questions 26-50 (comprehensive material)
